Tandem immunoaffinity purification of protein complexes from Caenorhabditis elegans

Tandem immunoaffinity purification of protein complexes from Caenorhabditis elegans.

[10] Protein- and Immunoaffinity Purification of Multiprotein Complexes

We have not had any problems using this method to purify proteins that are lethal on overexpression, including Abplp and Duolp. Although cells are dying during the induction, significant amounts of recombinant protein are produced. Because some proteins are sensitive to longer (>4 hr) induction times, it may be important to test protein expression at different time points during the induction. ...

Immunoaffinity purification of avermectin-binding proteins from the free-living nematode Caenorhabditis elegans and the fruitfly Drosophila melanogaster.

Avermectin-binding proteins from the free-living nematode worm Caenorhabditis elegans and from the fruitfly Drosophila melanogaster were purified to homogeneity via a three-step procedure. The binding proteins were covalently labelled using a radioactive photoaffinity probe and then partially purified on a Sephacryl S-300 gel-filtration column. The radiolabelled binding proteins were then purif...

Characterisation of protein microheterogeneity and protein complexes using on-chip immunoaffinity purification-mass spectrometry.

Post-translational modification of proteins may influence their interactions with other plasma proteins, as well as having an effect on many aspects of the metabolism of the protein, such as receptor binding, tissue uptake, degradation and excretion. Many post-translational modifications occur in a physiological context, while others are specific for certain diseases, which is why they are of d...

Affinity purification of protein complexes in C. elegans.

C. elegans is a powerful metazoan model system to address fundamental questions in cell and developmental biology. Research in C. elegans has traditionally focused on genetic, physiological, and cell biological approaches. However, C. elegans is also a facile system for biochemistry: worms are easy to grow in large quantities, the functionality of tagged fusion proteins can be assessed using mu...